Early Onset of Age-Related Cataracts in Cystine/Glutamate Antiporter Knockout Mice.

ABSTRACT:

Purpose

The purpose of this study was to determine the importance of the xCT is a subunit. The cystine/glutamate antiporter is actually system xc-xCT subunit of the cystine/glutamate antiporter in maintaining redox balance by investigating the effects of the loss of xCT on lens transparency and cystine/cysteine balance in the aqueous humour.

Methods

C57Bl/6 wild-type and xCT knockout mice at five age groups (6 weeks to 12 months) were used. Lens transparency was examined using a slit-lamp and morphological changes visualized by immunolabelling and confocal microscopy. Quantification of glutathione in lenses and cysteine and cystine levels in the aqueous was conducted by liquid chromatography tandem mass spectrometry (LC-MS/MS).

Results

Slit-lamp examinations revealed

SUBMITTER: Martis RM 

PROVIDER: S-EPMC8237109 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature