Differential and Altered Spatial Distribution of Complement Expression in Age-Related Macular Degeneration.

ABSTRACT:

Purpose

Dysregulation of the alternative complement pathway is a major pathogenic mechanism in age-related macular degeneration. We investigated whether locally synthesized complement components contribute to AMD by profiling complement expression in postmortem eyes with and without AMD.

Methods

AMD severity grade 1 to 4 was determined by analysis of postmortem acquired fundus images and hematoxylin and eosin stained histological sections. TaqMan (donor eyes n = 39) and RNAscope/in situ hybridization (n = 10) were performed to detect complement mRNA. Meso scale discovery assay and Western blot (n = 31) were used to measure complement protein levels.

Results

The levels of complement mRNA and protein expression were approximately 15- to 100-fold (P < 0.0001-0.001) highe

SUBMITTER: Demirs JT 

PROVIDER: S-EPMC8237111 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature