Assembly factors chaperone ribosomal RNA folding by isolating helical junctions that are prone to misfolding.

ABSTRACT: While RNAs are known to misfold, the underlying molecular causes have been mainly studied in fragments of biologically relevant larger RNAs. As these small RNAs are dominated by secondary structures, misfolding of these secondary structures remains the most-explored cause for global RNA misfolding. Conversely, how RNA chaperones function in a biological context to promote native folding beyond duplex annealing remains unknown. Here, in a combination of dimethylsulfate mutational profiling with sequencing (DMS-MaPseq), structural analyses, biochemical experiments, and yeast genetics, we show that three-helix junctions are prone to misfolding during assembly of the small ribosomal subunit in vivo. We identify ubiquitous roles for ribosome assembly factors in chaperoning their folding by prev

SUBMITTER: Huang H 

PROVIDER: S-EPMC8237570 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature