Feasibility of melting fingerprint obtained from ISSR-HRM curves for marine mammal species identification.

ABSTRACT: Currently, species identification of stranded marine mammals mostly relies on morphological features, which has inherent challenges. The use of genetic information for marine mammal species identification remains limited, therefore, new approaches that can contribute to a better monitoring of stranded species are needed. In that context, the ISSR-HRM method we have proposed offers a new approach for marine mammal species identification. Consequently, new approaches need to be developed to identify individuals at the species level. Eight primers of the ISSR markers were chosen for HRM analysis resulting in ranges of accuracy of 56.78-75.50% and 52.14-75.93% in terms of precision, while a degree of sensitivity of more than 80% was recorded when each single primer was used. The ISSR-HRM prime

SUBMITTER: Kriangwanich W 

PROVIDER: S-EPMC8237827 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature