Effect of confinement of horse heart cytochrome c and formate dehydrogenase from Candida boidinii on mesoporous carbons on their catalytic activity.

ABSTRACT: This study reports the immobilization of two biocatalysts (e.g., cytochrome c-Cyt c-and the non-metalloenzyme formate dehydrogenase from Candida boidinii-cbFDH) on a series of mesoporous carbons with controlled pore sizes. The catalytic activity of the nanoconfined proteins was correlated with the pore size distribution of the carbon materials used as supports. The electrochemical behaviour of nanoconfined Cyt c showed direct electron transfer electroactivity in pore sizes matching tightly the protein dimension. The pseudo-peroxidase activity towards H₂O₂ reduction was enhanced at pH 4.0, due to the protein conformational changes. For cbFDH, the reduction of CO₂ towards formic acid was evaluated for the nanoconfined protein, in the presence of nicotinamide

SUBMITTER: Hernandez-Ibanez N 

PROVIDER: S-EPMC8238777 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature