Spatial transcriptome profiling by MERFISH reveals fetal liver hematopoietic stem cell niche architecture.

ABSTRACT: The hematopoietic stem cell (HSC) niche has been extensively studied in bone marrow, yet a more systematic investigation into the microenvironment regulation of hematopoiesis in fetal liver is necessary. Here we investigate the spatial organization and transcriptional profile of individual cells in both wild type (WT) and Tet2^-/- fetal livers, by multiplexed error robust fluorescence in situ hybridization. We find that specific pairs of fetal liver cell types are preferentially positioned next to each other. Ligand-receptor signaling molecule pairs such as Kitl and Kit are enriched in neighboring cell types. The majority of HSCs are in direct contact with endothelial cells (ECs) in both WT and Tet2^-/- fetal livers. Loss of Tet2 increases the number of HSCs, and upregu

SUBMITTER: Lu Y 

PROVIDER: S-EPMC8238952 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature