Identification of the most suitable reference gene for gene expression studies with development and abiotic stress response in Bromus sterilis.

ABSTRACT: Bromus sterilis is an annual weedy grass, causing high yield losses in winter cereals. Frequent use of herbicides had led to the evolution of herbicide resistance in this species. Mechanisms underlying herbicide resistance in B. sterilis must be uncovered because this problem is becoming a global threat. qRT-PCR and the next-generation sequencing technologies can elucidate the resistance mechanisms. Although qRT-PCR can calculate precise fold changes, its preciseness depends on the expression of reference genes. Regardless of stable expression in any given condition, no gene can act as a universal reference gene. Hence, it is necessary to identify the suitable reference gene for each species. To our knowledge, there are no reports on the suitable reference gene in any brome species so far.

SUBMITTER: Sen MK 

PROVIDER: S-EPMC8238991 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature