GlycanGUI: Automated Glycan Annotation and Quantification Using Glucose Unit Index.

ABSTRACT: The retention time provides critical information for glycan annotation and quantification from the Liquid Chromatography Mass Spectrometry (LC-MS) data. However, the variation of the precise retention time of glycans is highly dependent on the experimental conditions such as the specific separating columns, MS instruments and/or the buffer used. This variation hampers the exploitation of retention time for the glycan annotation from LC-MS data, especially when inter-laboratory data are compared. To incorporate the retention time of glycan across experiments, Glucose Unit Index (GUI) can be computed using the dextrin ladder as internal standard. The retention time of glycans are then calibrated with respect to glucose units derived from dextrin ladders. Despite the successful application of

SUBMITTER: Zhang R 

PROVIDER: S-EPMC8239159 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature