Evaluation of spike protein antigens for SARS-CoV-2 serology.

ABSTRACT:

Background

Spike protein domains are being used in various serology-based assays to detect prior exposure to SARS-CoV-2 virus. However, there has been limited comparison of antibody titers against various spike protein antigens among COVID-19 infected patients.

Methods

We compared four spike proteins (RBD, S1, S2 and a stabilized spike trimer (ST)) representing commonly used antigens for their reactivity to human IgG antibodies using indirect ELISA in serum from COVID-19 patients and pre-2020 samples. ST ELISA was also compared against the EUROIMMUN IgG ELISA test. Further, we estimated time appropriate IgG and IgA seropositivity rates in COVID-19 patients using a panel of sera samples collected longitudinally from the day of onset of symptoms (DOS).

Results

Among the

SUBMITTER: Jagtap S 

PROVIDER: S-EPMC8239204 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature