Site-Specific N- and O-Glycosylation Analysis of Human Plasma Fibronectin.

ABSTRACT: Human plasma fibronectin is an adhesive protein that plays a crucial role in wound healing. Many studies had indicated that glycans might mediate the expression and functions of fibronectin, yet a comprehensive understanding of its glycosylation is still missing. Here, we performed a comprehensive N- and O-glycosylation mapping of human plasma fibronectin and quantified the occurrence of each glycoform in a site-specific manner. Intact N-glycopeptides were enriched by zwitterionic hydrophilic interaction chromatography, and N-glycosite sites were localized by the ¹⁸O-labeling method. O-glycopeptide enrichment and O-glycosite identification were achieved by an enzyme-assisted site-specific extraction method. An RP-LC-MS/MS system functionalized with collision-induced dissociation

SUBMITTER: Liu D 

PROVIDER: S-EPMC8239226 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature