Analysing Intercellular Communication in Astrocytic Networks Using "Astral".

ABSTRACT: Astrocytic networks are critically involved in regulating the activity of neuronal networks. However, a comprehensive and ready-to-use data analysis tool for investigating functional interactions between the astrocytes is missing. We developed the novel software package named "Astral" to analyse intercellular communication in astrocytic networks based on live-cell calcium imaging. Our method for analysing calcium imaging data does not require the assignment of regions of interest. The package contains two applications: the core processing pipeline for detecting and quantifying Ca⁺⁺ events, and the auxiliary visualization tool for controlling data quality. Our method allows for the network-wide quantification of Ca⁺⁺ events and the analysis of their intercellular propa

SUBMITTER: Dzyubenko E 

PROVIDER: S-EPMC8239356 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature