Overexpression of BnPCS1, a Novel Phytochelatin Synthase Gene From Ramie (Boehmeria nivea), Enhanced Cd Tolerance, Accumulation, and Translocation in Arabidopsis thaliana.

ABSTRACT: Phytochelatins (PCs) play important roles in the detoxification of and tolerance to heavy metals in plants. The synthesis of PCs is catalyzed by phytochelatin synthase (PCS), which is activated by heavy metal ions. In this study, we isolated a PCS gene, BnPCS1, from the bast fiber crop ramie (Boehmeria nivea) using the RACE (rapid amplification of cDNA ends) method. The full-length BnPCS1 cDNA is 1,949 bp in length with a 1,518 bp open reading frame (ORF) that encodes a 505 amino acid protein. The deduced BnPCS1 protein has a conserved N-terminus containing the catalytic triad Cys⁵⁸, His¹⁶⁴, Asp¹⁸², and a flexible C-terminal region containing a C³⁷¹C³⁷²QETC³⁷⁶VKC³⁷⁹ motif. The BnPCS1

SUBMITTER: Zhu S 

PROVIDER: S-EPMC8239399 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature