Quantitative Consequences of Protein Carriers in Immunopeptidomics and Tyrosine Phosphorylation MS² Analyses.

ABSTRACT: Utilizing a protein carrier in combination with isobaric labeling to "boost" the signal of other low-level samples in multiplexed analyses has emerged as an attractive strategy to enhance data quantity while minimizing protein input in mass spectrometry analyses. Recent applications of this approach include pMHC profiling and tyrosine phosphoproteomics, two applications that are often limited by large sample requirements. While including a protein carrier has been shown to increase the number of identifiable peptides in both applications, the impact of a protein carrier on quantitative accuracy remains to be thoroughly explored, particularly in relevant biological contexts where samples exhibit dynamic changes in abundance across peptides. Here, we describe two sets of analyses comparing M

SUBMITTER: Stopfer LE 

PROVIDER: S-EPMC8240026 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature