Programmable, Multiplexed DNA Circuits Supporting Clinically Relevant, Electrochemical Antibody Detection.

ABSTRACT: Current health emergencies have highlighted the need to have rapid, sensitive, and convenient platforms for the detection of specific antibodies. In response, we report here the design of an electrochemical DNA circuit that responds quantitatively to multiple specific antibodies. The approach employs synthetic antigen-conjugated nucleic acid strands that are rationally designed to induce a strand displacement reaction and release a redox reporter-modified strand upon the recognition of a specific target antibody. The approach is sensitive (low nanomolar detection limit), specific (no signal is observed in the presence of non-targeted antibodies), and selective (the platform can be employed in complex media, including 90% serum). The programmable nature of the strand displacement circuit ma

SUBMITTER: Bracaglia S 

PROVIDER: S-EPMC8240086 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature