Lung but not brain cancer cell malignancy inhibited by commonly used anesthetic propofol during surgery: Implication of reducing cancer recurrence risk.

ABSTRACT:

Introduction

Intravenous anesthesia with propofol was reported to improve cancer surgical outcomes when compared with inhalational anesthesia. However, the underlying molecular mechanisms largely remain unknown.

Objectives

The anti-tumor effects of propofol and the possible underlying mechanism including altered metabolic and signaling pathways were studied in the current study.

Methods

The cell viability, proliferation, migration, and invasion of cancer cells were analyzed with CCK-8, Ki-67 staining, wound healing, and Transwell assay, respectively. The protein changes were analyzed with Western blot and immunofluorescent staining. The metabolomics alteration was studied with ¹H-NMR spectroscopy. The gene expression regulations were analyzed with PCR gene

SUBMITTER: Hu C 

PROVIDER: S-EPMC8240101 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature