Sleep/wake calcium dynamics, respiratory function, and ROS production in cardiac mitochondria.

ABSTRACT:

Introduction

Incidents of myocardial infarction and sudden cardiac arrest vary with time of the day, but the mechanism for this effect is not clear. We hypothesized that diurnal changes in the ability of cardiac mitochondria to control calcium homeostasis dictate vulnerability to cardiovascular events.

Objectives

Here we investigate mitochondrial calcium dynamics, respiratory function, and reactive oxygen species (ROS) production in mouse heart during different phases of wake versus sleep periods.

Methods

We assessed time-of-the-day dependence of calcium retention capacity of isolated heart mitochondria from young male C57BL6 mice. Rhythmicity of mitochondrial-dependent oxygen consumption, ROS production and transmembrane potential in homogenates were explored using t

SUBMITTER: Abdel-Rahman EA 

PROVIDER: S-EPMC8240107 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature