Oxygen concentration affects de novo DNA methylation and transcription in in vitro cultured oocytes.

ABSTRACT:

Background

Reproductive biology methods rely on in vitro follicle cultures from mature follicles obtained by hormonal stimulation for generating metaphase II oocytes to be fertilised and developed into a healthy embryo. Such techniques are used routinely in both rodent and human species. DNA methylation is a dynamic process that plays a role in epigenetic regulation of gametogenesis and development. In mammalian oocytes, DNA methylation establishment regulates gene expression in the embryos. This regulation is particularly important for a class of genes, imprinted genes, whose expression patterns are crucial for the next generation. The aim of this work was to establish an in vitro culture system for immature mouse oocytes that will allow manipulation of specific factors for a deep

SUBMITTER: Naillat F 

PROVIDER: S-EPMC8240245 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature