MicroRNAs secreted by human embryos could be potential biomarkers for clinical outcomes of assisted reproductive technology.

ABSTRACT:

Introduction

MicroRNAs (miRNAs) are important regulators of many biological functions, including embryo implantation and development. Recently, it has been reported that miRNAs in biofluids are predictive for physiological and pathological processes.

Objectives

In this study, we aim to investigate whether the miRNAs secreted by human embryos in culture medium can be used as embryonic biomarkers.

Methods

The culture media were prospectively collected from embryos of patients at reproductive medicine center with informed consent. A high-throughput miRNA sequencing method was applied to detect the miRNA profiles in the human embryo culture media. After bioinformatics analysis and screening of differentially expressed miRNAs, quantitative real-time polymerase chain reacti

SUBMITTER: Fang F 

PROVIDER: S-EPMC8240345 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature