Emergency myelopoiesis contributes to immune cell exhaustion and pulmonary vascular remodelling.

ABSTRACT:

Background and purpose

Pulmonary hypertension (PH) secondary to chronic lung disease (World Health Organization Group 3 PH) is deadly, with lung transplant being the only available long-term treatment option. Myeloid-derived cells are known to affect progression of both pulmonary fibrosis and PH, although the mechanism of action is unknown. Therefore, we investigated the effect of myeloid cell proliferation induced by emergency myelopoiesis on development of PH and therapy directed against programmed death-ligand 1 (PD-L1), expressed by myeloid cells in prevention of pulmonary vascular remodelling.

Experimental approach

LysM.Cre-DTR ("mDTR") mice were injected with bleomycin (0.018 U·g^-1 , i.p.) while receiving either vehicle or diphtheria toxin (DT; 100 ng, i.p.)

SUBMITTER: Fu C 

PROVIDER: S-EPMC8240454 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature