Fluorescence lifetime predicts performance of voltage sensitive fluorophores in cardiomyocytes and neurons.

ABSTRACT: Voltage imaging with fluorescent indicators offers a powerful complement to traditional electrode or Ca²⁺-imaging approaches for monitoring electrical activity. Small molecule fluorescent indicators present the unique opportunity for exquisite control over molecular structure, enabling detailed investigations of structure/function relationships. In this paper, we tune the conjugation between aniline donors and aromatic π systems within the context of photoinduced electron transfer (PeT) based voltage indicators. We describe the design and synthesis of four new voltage-sensitive fluorophores (VoltageFluors, or VFs). Three of these dyes have higher relative voltage sensitivities (ΔF/F) than the previously-reported indicator, VF2.1.Cl. We pair these new indicators with existing VFs

SUBMITTER: Boggess SC 

PROVIDER: S-EPMC8240514 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature