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Microbial exposure during early human development primes fetal immune cells.

ABSTRACT: The human fetal immune system begins to develop early during gestation; however, factors responsible for fetal immune-priming remain elusive. We explored potential exposure to microbial agents in utero and their contribution toward activation of memory T cells in fetal tissues. We profiled microbes across fetal organs using 16S rRNA gene sequencing and detected low but consistent microbial signal in fetal gut, skin, placenta, and lungs in the 2^nd trimester of gestation. We identified several live bacterial strains including Staphylococcus and Lactobacillus in fetal tissues, which induced in vitro activation of memory T cells in fetal mesenteric lymph node, supporting the role of microbial exposure in fetal immune-priming. Finally, using SEM and RNA-ISH, we visualized discrete localization of bacteria-like structures and eubacterial-RNA within 14^th weeks fetal gut lumen. These findings indicate selective presence of live microbes in fetal organs during the 2^nd trimester of gestation and have broader implications toward the establishment of immune competency and priming before birth.

SUBMITTER: Mishra A

PROVIDER: S-EPMC8240556 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Microbial exposure during early human development primes fetal immune cells.

Mishra Archita A Lai Ghee Chuan GC Yao Leong Jing LJ Aung Thet Tun TT Shental Noam N Rotter-Maskowitz Aviva A Shepherdson Edwin E Singh Gurmit Singh Naranjan GSN Pai Rhea R Shanti Adhika A Wong Regina Men Men RMM Lee Andrea A Khyriem Costerwell C Dutertre Charles Antoine CA Chakarov Svetoslav S Srinivasan K G KG Shadan Nurhidaya Binte NB Zhang Xiao-Meng XM Khalilnezhad Shabnam S Cottier Fabien F Tan Alrina Shin Min ASM Low Gillian G Chen Phyllis P Fan Yiping Y Hor Pei Xiang PX Lee Avery Khoo May AKM Choolani Mahesh M Vermijlen David D Sharma Ankur A Fuks Garold G Straussman Ravid R Pavelka Norman N Malleret Benoit B McGovern Naomi N Albani Salvatore S Chan Jerry Kok Yen JKY Ginhoux Florent F

Cell 20210601 13

The human fetal immune system begins to develop early during gestation; however, factors responsible for fetal immune-priming remain elusive. We explored potential exposure to microbial agents in utero and their contribution toward activation of memory T cells in fetal tissues. We profiled microbes across fetal organs using 16S rRNA gene sequencing and detected low but consistent microbial signal in fetal gut, skin, placenta, and lungs in the 2<sup>nd</sup> trimester of gestation. We identified ...[more]

PMID: 34077752

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Project description:Positional information on the shoulder girdle (the clavicle and scapula) is important for a better understanding of the function of the upper limb in the locomotive system as well as its associated disease pathogenesis. However, such data are limited except for information on the axial position of the scapula. Here, we describe a three-dimensional reconstruction of the shoulder girdle including the clavicle and scapula, and its relationship to different landmarks in the body. Thirty-six human fetal specimens (crown-rump length range: 7.6-225 mm) from the Kyoto Collection were used for this study. The morphogenesis and three-dimensional position of the shoulder girdle were analyzed with phase-contrast X-ray computed tomography and magnetic resonance imaging. We first detected the scapula body along with the coracoid and humeral head at Carnegie stage 18; however, the connection between the body and coracoid was not confirmed at this stage. During development, all landmarks on the shoulder girdle remained at the same axial position except for the inferior angle, which implies that the scapula enlarged in the caudal direction and reached the adult axial position in the fetal period. The scapula body was rotated internally and in the upward direction at the initiation of morphogenesis, but in the fetal period the scapula body was different than that in the adult position. The shoulder girdle was located at the ventral side of the vertebrae at the time of initial morphogenesis, but changed its position to the lateral side of the vertebrae in the late embryonic and fetal periods. Such a unique position of the shoulder girdle may contribute to the stage-specific posture of the upper limb. Adequate internal and upward rotation of the scapula could help in reducing the shoulder width, thereby facilitating childbirth. The data presented in this study can be used as normal morphometric references for shoulder girdle evaluations in the embryonic and fetal periods.

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Microbial exposure during early human development primes fetal immune cells.

Publications

Microbial exposure during early human development primes fetal immune cells.

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