A Synthetic Riboswitch to Regulate Haloarchaeal Gene Expression.

ABSTRACT: In recent years, synthetic riboswitches have become increasingly important to construct genetic circuits in all three domains of life. In bacteria, synthetic translational riboswitches are often employed that modulate gene expression by masking the Shine-Dalgarno (SD) sequence in the absence or presence of a cognate ligand. For (halo-)archaeal translation, a SD sequence is not strictly required. The application of synthetic riboswitches in haloarchaea is therefore limited so far, also because of the molar intracellular salt concentrations found in these microbes. In this study, we applied synthetic theophylline-dependent translational riboswitches in the archaeon Haloferax volcanii. The riboswitch variants A through E and E^∗ were chosen since they not only mask the SD seq

SUBMITTER: Born J 

PROVIDER: S-EPMC8241225 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature