Significance of PD1 Alternative Splicing in Celiac Disease as a Novel Source for Diagnostic and Therapeutic Target.

ABSTRACT:

Background

We have focused on the alteration of the PD-1/PD-L1 pathway in celiac disease and discussed the roles of the PD1 pathway in regulating the immune response. We explored the idea that the altered mRNA splicing process in key regulatory proteins could represent a novel source to identify diagnostic, prognostic, and therapeutic targets in celiac disease.

Methods

We characterized the PD1 mRNA variants' profile in CD patients and in response to gluten peptides' incubation after in vitro experiments. Total RNA from whole blood was isolated, and the coding region of the human PD-1 mRNA was amplified by cDNA PCR.

Results

PCR amplification of the human PD-1 coding sequence revealed an association between the over-expression of the sPD-1 protein and the

SUBMITTER: Ponce de Leon C 

PROVIDER: S-EPMC8242946 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature