Liposomes embedded within fibrin gels facilitate localized macrophage manipulations within nerve.

ABSTRACT:

Background

Understanding the role of macrophages at discrete spatial locations during nerve regeneration after injury is important. But, methodologies that systemically manipulate macrophages can obscure their roles within discrete spatial locations within nerve.

New method

Liposomes were embedded within fibrin gels to construct a delivery system that facilitated macrophage-specific manipulations at a sole spatial region, as macrophages accumulated within the fibrin. Clodronate liposomes were characterized for their toxicity to specific cells composing nerve in vitro, then tested for macrophage-specific depletion in vivo. This delivery system using clodronate liposomes was used to repair a mouse sciatic nerve gap to evaluate its efficacy and effects.

Result

Clodronate

SUBMITTER: Pan D 

PROVIDER: S-EPMC8243038 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature