RAGE-mediated functional DNA methylated modification contributes to cigarette smoke-induced airway inflammation in mice.

ABSTRACT: Our previous study indicated knockout of receptor for advanced glycation end-products (RAGE) significantly attenuated cigarette smoke (CS)-induced airway inflammation in mice. In the present study, we aim to further detect the mediatory effects of RAGE in DNA methylated modification in CS-induced airway inflammation. Lung tissues from the CS-exposed mouse model of airway inflammation were collected for profiling of DNA methylation by liquid hybridization capture-based bisulfite sequencing, which were used for conjoint analysis with our previous data of gene expression by cDNA microarray to identify functional methylated genes, as well as hub genes selected by protein-protein interaction (PPI) network analysis, and functional enrichment analyses were then performed. After RAGE knockout, 90

SUBMITTER: Li P 

PROVIDER: S-EPMC8243334 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature