Double overexpression of miR-19a and miR-20a in induced pluripotent stem cell-derived mesenchymal stem cells effectively preserves the left ventricular function in dilated cardiomyopathic rat.

ABSTRACT:

Background

This study tested the hypothesis that double overexpression of miR-19a and miR-20a (dOex-mIRs) in human induced pluripotent stem cell (iPS)-derived mesenchymal stem cells (MSCs) effectively preserved left ventricular ejection fraction (LVEF) in dilated cardiomyopathy (DCM) (i.e., induced by doxorubicin) rat.

Methods and results

In vitro study was categorized into groups G1 (iPS-MSC), G2 (iPS-MSC^dOex-mIRs), G3 (iPS-MSC + H₂O₂/100uM), and G4 (iPS-MSC^dOex-mIRs + H₂O₂/100uM). The in vitro results showed the cell viability was significantly lower in G3 than in G1 and G2, and that was reversed in G4 but it showed no difference between G1/G2 at time points of 6 h/24 h/48 h, whereas the flow cytometry of in

SUBMITTER: Sheu JJ 

PROVIDER: S-EPMC8243466 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature