Single cell transcriptome atlas of mouse mammary epithelial cells across development.

ABSTRACT:

Background

Heterogeneity within the mouse mammary epithelium and potential lineage relationships have been recently explored by single-cell RNA profiling. To further understand how cellular diversity changes during mammary ontogeny, we profiled single cells from nine different developmental stages spanning late embryogenesis, early postnatal, prepuberty, adult, mid-pregnancy, late-pregnancy, and post-involution, as well as the transcriptomes of micro-dissected terminal end buds (TEBs) and subtending ducts during puberty.

Methods

The single cell transcriptomes of 132,599 mammary epithelial cells from 9 different developmental stages were determined on the 10x Genomics Chromium platform, and integrative analyses were performed to compare specific time points.

Results

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SUBMITTER: Pal B 

PROVIDER: S-EPMC8243869 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature