Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid.

ABSTRACT: Suspension culture is an important method used in the industrial preparation of pluripotent stem cells (PSCs), for regenerative therapy and drug screening. Generally, a suspension culture requires agitation to keep PSC aggregates suspended and to promote mass transfer, but agitation also causes cell damage. In this study, we investigated the use of a Bingham plastic fluid, supplemented with a polysaccharide-based polymer, to preserve PSCs from cell damage in suspension culture. Rheometric analysis showed that the culture medium gained yield stress and became a Bingham plastic fluid, after supplementation with the polymer FP003. A growth/death analysis revealed that 2 days of aggregate formation and 2 days of suspension in the Bingham plastic medium improved cell growth and prevented cell d

SUBMITTER: Horiguchi I 

PROVIDER: S-EPMC8244041 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature