Calmodulin complexes with brain and muscle creatine kinase peptides.

ABSTRACT: Calmodulin (CaM) is a ubiquitous Ca²⁺ sensing protein that binds to and modulates numerous target proteins and enzymes during cellular signaling processes. A large number of CaM-target complexes have been identified and structurally characterized, revealing a wide diversity of CaM-binding modes. A newly identified target is creatine kinase (CK), a central enzyme in cellular energy homeostasis. This study reports two high-resolution X-ray structures, determined to 1.24 ​Å and 1.43 ​Å resolution, of calmodulin in complex with peptides from human brain and muscle CK, respectively. Both complexes adopt a rare extended binding mode with an observed stoichiometry of 1:2 CaM:peptide, confirmed by isothermal titration calorimetry, suggesting that each CaM domain independently binds one

SUBMITTER: Sprenger J 

PROVIDER: S-EPMC8244255 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature