Saccharomyces cerevisiae as a superior host for overproduction of prokaryotic integral membrane proteins.

ABSTRACT: Integral membrane proteins (IMPs) constitute ~30% of all proteins encoded by the genome of any organism and Escherichia coli remains the first-choice host for recombinant production of prokaryotic IMPs. However, the expression levels of prokaryotic IMPs delivered by this bacterium are often low and overproduced targets often accumulate in inclusion bodies. The targets are therefore often discarded to avoid an additional and inconvenient refolding step in the purification protocol. Here we compared expression of five prokaryotic (bacterial and archaeal) IMP families in E. coli and Saccharomyces cerevisiae. We demonstrate that our S. cerevisiae-based production platform is superior in expression of four investigated IMPs, overall being able to deliver high quantit

SUBMITTER: Preisler SS 

PROVIDER: S-EPMC8244417 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature