The influence of the R47H triggering receptor expressed on myeloid cells 2 variant on microglial exosome profiles.

ABSTRACT: Variants in the triggering receptor expressed on myeloid cells 2 gene are linked with an increased risk of dementia, in particular the R47H^het triggering receptor expressed on myeloid cells 2 variant is linked to late-onset Alzheimer's disease. Using human induced pluripotent stem cells-derived microglia, we assessed whether variations in the dynamics of exosome secretion, including their components, from these cells might underlie some of this risk. We found exosome size was not altered between common variant controls and R47H^het variants, but the amount and constitution of exosomes secreted were different. Exosome quantities were rescued by incubation with an ATP donor or with lipids via a phosphatidylserine triggering receptor expressed on myeloid cells 2 ligand. F

SUBMITTER: Mallach A 

PROVIDER: S-EPMC8244649 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature