Introducing differential RNA-seq mapping to track the early infection phase for Pseudomonas phage ɸKZ.

ABSTRACT: As part of the ongoing renaissance of phage biology, more phage genomes are becoming available through DNA sequencing. However, our understanding of the transcriptome architecture that allows these genomes to be expressed during host infection is generally poor. Transcription start sites (TSSs) and operons have been mapped for very few phages, and an annotated global RNA map of a phage - alone or together with its infected host - is not available at all. Here, we applied differential RNA-seq (dRNA-seq) to study the early, host takeover phase of infection by assessing the transcriptome structure of Pseudomonas aeruginosa jumbo phage ɸKZ, a model phage for viral genetics and structural research. This map substantially expands the number of early expressed viral genes, defining TSSs th

SUBMITTER: Wicke L 

PROVIDER: S-EPMC8244752 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature