The role of internal transcribed spacer 2 secondary structures in classifying mycoparasitic Ampelomyces.

ABSTRACT: Many fungi require specific growth conditions before they can be identified. Direct environmental DNA sequencing is advantageous, although for some taxa, specific primers need to be used for successful amplification of molecular markers. The internal transcribed spacer region is the preferred DNA barcode for fungi. However, inter- and intra-specific distances in ITS sequences highly vary among some fungal groups; consequently, it is not a solely reliable tool for species delineation. Ampelomyces, mycoparasites of the fungal phytopathogen order Erysiphales, can have ITS genetic differences up to 15%; this may lead to misidentification with other closely related unknown fungi. Indeed, Ampelomyces were initially misidentified as other pycnidial mycoparasites, but subsequent research showed th

SUBMITTER: Prahl RE 

PROVIDER: S-EPMC8244850 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature