Measurement of Telomere Length for Longitudinal Analysis: Implications of Assay Precision.

ABSTRACT: Researchers increasingly wish to test hypotheses concerning the impact of environmental or disease exposures on telomere length (TL), and they use longitudinal study designs to do so. In population studies, TL is usually measured with a quantitative polymerase chain reaction (qPCR)-based method. This method has been validated by calculating its correlation with a gold standard method such as Southern blotting (SB) in cross-sectional data sets. However, in a cross-section, the range of true variation in TL is large, and measurement error is introduced only once. In a longitudinal study, the target variation of interest is small, and measurement error is introduced at both baseline and follow-up. In this paper, we present results from a small data set (n = 20) in which leukocyte TL was measu

SUBMITTER: Nettle D 

PROVIDER: S-EPMC8245883 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature