Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

ABSTRACT: Ageing-related processes are largely conserved, with simple organisms remaining the main platform to discover and dissect new ageing-associated genes. Yeasts provide potent model systems to study cellular ageing owing their amenability to systematic functional assays under controlled conditions. Even with yeast cells, however, ageing assays can be laborious and resource-intensive. Here we present improved experimental and computational methods to study chronological lifespan in Schizosaccharomyces pombe. We decoded the barcodes for 3206 mutants of the latest gene-deletion library, enabling the parallel profiling of ~700 additional mutants compared to previous screens. We then applied a refined method of barcode sequencing (Bar-seq), addressing technical and statistical issues raised

SUBMITTER: Romila CA 

PROVIDER: S-EPMC8246024 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature