Long-lived lanthanide emission via a pH-sensitive and switchable LRET complex.

ABSTRACT: Lanthanide-based luminescence resonance energy transfer (LRET) can be used as a tool to enhance lanthanide emission for time-resolved cellular imaging applications. By shortening lanthanide emission lifetimes whilst providing an alternative radiative pathway to the formally forbidden, weak lanthanide-only emission, the photon flux of such systems is increased. With this aim in mind, we investigated energy transfer in differently spaced donor-acceptor terbium-rhodamine pairs with the LRET "on" (low pH) and LRET "off" (high pH). Results informed the design, preparation and characterisation of a compound containing terbium, a spectrally-matched pH-responsive fluorophore and a receptor-targeting group. By combining these elements, we observed switchable LRET, where the targeting group sensitis

SUBMITTER: Boltersdorf T 

PROVIDER: S-EPMC8246121 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature