Transient exposure to elevated glucose levels causes persistent changes in dermal microvascular endothelial cell responses to injury.

ABSTRACT:

Background

The purpose of this study was to determine whether elevated glucose can induce a dermal microvascular endothelial cell metabolic memory, thus affecting angiogenesis in the repair process of mammalian cutaneous wound. We hypothesized that transient elevated glucose levels cause sustained alteration of endothelial cell responses to injury and persistent epigenetic changes in gene expression.

Methods

Human dermal microvascular endothelial cells were exposed to experimental conditions with or without 30 mM D-glucose. The control group was maintained at 5 mM D-glucose; while in the transient glucose group, after being exposed to 30 mM D-glucose for two days, then being put under the control conditions during the experiment. Besides, in the whole process of the experime

SUBMITTER: Wang Q 

PROVIDER: S-EPMC8246238 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature