Glycan-protein cross-linking mass spectrometry reveals sialic acid-mediated protein networks on cell surfaces.

ABSTRACT: A cross-linking method is developed to elucidate glycan-mediated interactions between membrane proteins through sialic acids. The method provides information on previously unknown extensive glycomic interactions on cell membranes. The vast majority of membrane proteins are glycosylated with complicated glycan structures attached to the polypeptide backbone. Glycan-protein interactions are fundamental elements in many cellular events. Although significant advances have been made to identify protein-protein interactions in living cells, only modest advances have been made on glycan-protein interactions. Mechanistic elucidation of glycan-protein interactions has thus far remained elusive. Therefore, we developed a cross-linking mass spectrometry (XL-MS) workflow to directly identify glycan-pr

SUBMITTER: Xie Y 

PROVIDER: S-EPMC8246274 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature