Translatomic analysis of regenerating and degenerating spinal motor neurons in injury and ALS.

ABSTRACT: The neuromuscular junction is a synapse critical for muscle strength and coordinated motor function. Unlike CNS injuries, motor neurons mount robust regenerative responses after peripheral nerve injuries. Conversely, motor neurons selectively degenerate in diseases such as amyotrophic lateral sclerosis (ALS). To assess how these insults affect motor neurons in vivo, we performed ribosomal profiling of mouse motor neurons. Motor neuron-specific transcripts were isolated from spinal cords following sciatic nerve crush, a model of acute injury and regeneration, and in the SOD1^G93A ALS model. Of the 267 transcripts upregulated after nerve crush, 38% were also upregulated in SOD1^G93A motor neurons. However, most upregulated genes in injured and ALS motor neurons wer

SUBMITTER: Shadrach JL 

PROVIDER: S-EPMC8246596 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature