MsGBS: A new high-throughput approach to quantify the relative species abundance in root samples of multispecies plant communities.

ABSTRACT: Plant interactions are as important belowground as aboveground. Belowground plant interactions are however inherently difficult to quantify, as roots of different species are difficult to disentangle. Although for a couple of decades molecular techniques have been successfully applied to quantify root abundance, root identification and quantification in multispecies plant communities remains particularly challenging. Here we present a novel methodology, multispecies genotyping by sequencing (msGBS), as a next step to tackle this challenge. First, a multispecies meta-reference database containing thousands of gDNA clusters per species is created from GBS derived High Throughput Sequencing (HTS) reads. Second, GBS derived HTS reads from multispecies root samples are mapped to this meta-refer

SUBMITTER: Wagemaker CAM 

PROVIDER: S-EPMC8246947 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature