Protocol development for somatic embryogenesis, SSR markers and genetic modification of Stipagrostis pennata (Trin.) De Winter.

ABSTRACT:

Background

Stipagrostis pennata (Trin.) De Winter is an important species for fixing sand in shifting and semi-fixed sandy lands, for grazing, and potentially as a source of lignocellulose fibres for pulp and paper industry. The seeds have low viability, which limits uses for revegetation. Somatic embryogenesis offers an alternative method for obtaining large numbers of plants from limited seed sources.

Results

A protocol for plant regeneration from somatic embryos of S. pennata was developed. Somatic embryogenesis was induced on Murashige & Skoog (MS) medium supplemented with 3 mg·L^-1 2,4-D subsequently shoots were induced on MS medium and supplemented with 5 mg·L^-1 zeatin riboside. The highest shoots induction was obtained when embryogenic callus deri

SUBMITTER: Asadi-Aghbolaghi M 

PROVIDER: S-EPMC8247082 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature