Regulation of exosome secretion by cellular retinoic acid binding protein 1 contributes to systemic anti-inflammation.

ABSTRACT:

Background

Intercellular communications are important for maintaining normal physiological processes. An important intercellular communication is mediated by the exchange of membrane-enclosed extracellular vesicles. Among various vesicles, exosomes can be detected in a wide variety of biological systems, but the regulation and biological implication of exosome secretion/uptake remains largely unclear.

Methods

Cellular retinoic acid (RA) binding protein 1 (Crabp1) knockout (CKO) mice were used for in vivo studies. Extracellular exosomes were monitored in CKO mice and relevant cell cultures including embryonic stem cell (CJ7), macrophage (Raw 264.7) and hippocampal cell (HT22) using Western blot and flow cytometry. Receptor Interacting Protein 140 (RIP140) was depleted by Cris

SUBMITTER: Lin YW 

PROVIDER: S-EPMC8247179 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature