Identification of Pru du 6 as a potential marker allergen for almond allergy.

ABSTRACT:

Background

Oral food challenges have demonstrated that diagnosis of almond allergy based on extract-sIgE tests displays low specificity. Molecular allergy diagnosis is expected to improve accuracy, but its value in diagnosing almond allergy remains unknown. The aim of this study was to identify relevant almond allergens and examine their ability to improve almond allergy diagnosis.

Methods

IgE-reactive proteins were purified from almond kernels. IgE binding to almond extract and the allergens was analyzed by quantitative ELISA using sera from 18 subjects with a proven almond allergy. The control group consisted of sera from 18 subjects allergic to peanut and/or tree nuts but tolerant to almond.

Results

Three IgE-binding proteins were identified: legumin (Pru du 6), al

SUBMITTER: Kabasser S 

PROVIDER: S-EPMC8247360 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature