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Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia.

ABSTRACT: Given its myeloid-restricted expression, myeloperoxidase (MPO) is typically used for lineage assignment (myeloid vs. lymphoid) during acute leukaemia (AL) diagnostics. In the present study, a robust flow cytometric definition for MPO positivity was established based on the standardised EuroFlow protocols, the standardised Acute Leukaemia Orientation Tube and 1734 multicentre AL cases (with confirmed assay stability). The best diagnostic performance was achieved by defining MPO positivity as ≥20% of the AL cells exceeding a lymphocyte-based threshold. The methodology employed should be applicable to any form of standardised flow cytometry.

SUBMITTER: Bras AE

PROVIDER: S-EPMC8247412 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia.

Bras Anne E AE Osmani Zgjim Z de Haas Valerie V Jongen-Lavrencic Mojca M Te Marvelde Jeroen G JG Zwaan C Michel CM Mejstrikova Ester E Fernandez Paula P Szczepanski Tomasz T Orfao Alberto A van Dongen Jacques J M JJM van der Velden Vincent H J VHJ

British journal of haematology 20201107 5

Given its myeloid-restricted expression, myeloperoxidase (MPO) is typically used for lineage assignment (myeloid vs. lymphoid) during acute leukaemia (AL) diagnostics. In the present study, a robust flow cytometric definition for MPO positivity was established based on the standardised EuroFlow protocols, the standardised Acute Leukaemia Orientation Tube and 1734 multicentre AL cases (with confirmed assay stability). The best diagnostic performance was achieved by defining MPO positivity as ≥20% ...[more]

PMID: 33161592

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