HAMLET a human milk protein-lipid complex induces a pro-inflammatory phenotype of myeloid cells.

ABSTRACT: HAMLET is a protein-lipid complex with a specific and broad bactericidal and tumoricidal activity, that lacks cytotoxic activity against healthy cells. In this study, we show that HAMLET also has general immune-stimulatory effects on primary human monocyte-derived dendritic cells and macrophages (Mo-DC and Mo-M) and murine RAW264.7 macrophages. HAMLET, but not its components alpha-lactalbumin or oleic acid, induces mature CD14^low/- CD83⁺ Mo-DC and M1-like CD14⁺ CD86⁺⁺ Mo-M surface phenotypes. Concomitantly, inflammatory mediators, including IL-2, IL-6, IL-10, IL-12 and MIP-1α, were released in the supernatant of HAMLET-stimulated cells, indicating a mainly pro-inflammatory phenotype. The HAMLET-induced phenotype was mediated by calcium, NFκB and

SUBMITTER: Vansarla G 

PROVIDER: S-EPMC8248127 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature