Astrocytes in stress accumulate lipid droplets.

ABSTRACT: When the brain is in a pathological state, the content of lipid droplets (LDs), the lipid storage organelles, is increased, particularly in glial cells, but rarely in neurons. The biology and mechanisms leading to LD accumulation in astrocytes, glial cells with key homeostatic functions, are poorly understood. We imaged fluorescently labeled LDs by microscopy in isolated and brain tissue rat astrocytes and in glia-like cells in Drosophila brain to determine the (sub)cellular localization, mobility, and content of LDs under various stress conditions characteristic for brain pathologies. LDs exhibited confined mobility proximal to mitochondria and endoplasmic reticulum that was attenuated by metabolic stress and by increased intracellular Ca²⁺ , likely to enhance the LD-organelle

SUBMITTER: Smolic T 

PROVIDER: S-EPMC8248329 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature