Local production of lactate, ribose phosphate, and amino acids within human triple-negative breast cancer.

ABSTRACT:

Background

Upregulated glucose metabolism is a common feature of tumors. Glucose can be broken down by either glycolysis or the oxidative pentose phosphate pathway (oxPPP). The relative usage within tumors of these catabolic pathways remains unclear. Similarly, the extent to which tumors make biomass precursors from glucose, versus take them up from the circulation, is incompletely defined.

Methods

We explore human triple negative breast cancer (TNBC) metabolism by isotope tracing with [1,2-¹³C]glucose, a tracer that differentiates glycolytic versus oxPPP catabolism and reveals glucose-driven anabolism. Patients enrolled in clinical trial NCT03457779 and received IV infusion of [1,2-¹³C]glucose during core biopsy of their primary TNBC. Tumor samples wer

SUBMITTER: Ghergurovich JM 

PROVIDER: S-EPMC8248508 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature