A Hyperthermophilic Argonaute From Ferroglobus placidus With Specificity on Guide Binding Pattern.

ABSTRACT: Argonaute proteins (Agos) from thermophilic archaea are involved in several important processes, such as host defense and DNA replication. The catalytic mechanism of Ago from different microbes with great diversity and genome editing potential is attracting increasing attention. Here, we describe an Argonaute from hyperthermophilic Ferroglobus placidus (FpAgo), with a typical DNA-guided DNA endonuclease activity but adopted with only a short guide 15-20 nt length rather than a broad guide selectivity for reported Agos. FpAgo performed the precise cleavage of phosphodiester bonds between 10 and 11 nt on the target strand (counting from the guide strand) guided strictly by 5'-phosphorylated DNA at temperatures ranging from 75 to 99°C. The cleavage activity was regulated

SUBMITTER: Guo X 

PROVIDER: S-EPMC8248672 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature