High-Resolution pO₂ Imaging Improves Quantification of the Hypoxic Fraction in Tumors During Radiation Therapy.

ABSTRACT:

Purpose

The extreme microscopic heterogeneity of tumors makes it difficult to characterize tumor hypoxia. We evaluated how changes in the spatial resolution of oxygen imaging could alter measures of tumor hypoxia and their correlation to radiation therapy response.

Methods and materials

Cherenkov-Excited Luminescence Imaging in combination with an oxygen probe, Oxyphor PtG4 was used to directly image tumor pO₂ distributions with 0.2 mm spatial resolution at the time of radiation delivery. These pO₂ images were analyzed with variations of reduced spatial resolution from 0.2 mm to 5 mm, to investigate the influence of how reduced imaging spatial resolution would affect the observed tumor hypoxia. As an in vivo validation test, mice bearing tumor xenograft

SUBMITTER: Cao X 

PROVIDER: S-EPMC8249085 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature