Functionally distinct POMC-expressing neuron subpopulations in hypothalamus revealed by intersectional targeting.

ABSTRACT: Pro-opiomelanocortin (POMC)-expressing neurons in the arcuate nucleus of the hypothalamus represent key regulators of metabolic homeostasis. Electrophysiological and single-cell sequencing experiments have revealed a remarkable degree of heterogeneity of these neurons. However, the exact molecular basis and functional consequences of this heterogeneity have not yet been addressed. Here, we have developed new mouse models in which intersectional Cre/Dre-dependent recombination allowed for successful labeling, translational profiling and functional characterization of distinct POMC neurons expressing the leptin receptor (Lepr) and glucagon like peptide 1 receptor (Glp1r). Our experiments reveal that POMC^Lepr+ and POMC^Glp1r+ neurons represent largely nonoverlapping subpo

SUBMITTER: Biglari N 

PROVIDER: S-EPMC8249241 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature